FAQs
Any questions?
The development of a new PGT-M test usually requires 3 weeks starting from the date that Juno receives all of the necessary DNA samples and paperwork, but can take up to 6 weeks in some cases. Once Juno has confirmed that the test is ready, the IVF cycle can begin. The embryos produced can be biopsied and the specimens sent to Juno. PGT[M]Seq results are generally available 9 days after Juno receives the embryo biopsy samples (although can take up to 14 days).
We aim to make working with Juno Genetics as easy as possible. Juno is committed to supporting the clinics it works with, providing guidance at all stages of the PGT process. We are available seven days a week to assist with queries or to provide advice. At Juno we appreciate that different clinics may have unique needs and our policy is one of flexibility, where we adapt to the specific requirements of each clinic. If your clinic already offers PGT in collaboration with another genetics laboratory, then it is likely that very few significant changes, if any, will be necessary. Please contact Juno Genetics and we will be happy to walk you through the enrolment procedure, provide all necessary documentation and answer any questions you might have. If your clinic is new to PGT, Juno can help by providing valuable information and documentation to get you started. Juno can also assist with training and evaluation of procedures, making sure that all elements of the PGT procedure are optimised and working well, prior to the launch of a clinical service. Please contact supportuk@junogenetics.com to begin working with Juno.
If the proportion of samples that fail to give a PGT result is consistently higher than expected, this is almost always due to a problem with technique used for the cell washing and loading or, less commonly, with the embryo biopsy method. Scientists at Juno Genetics have decades of experience working with clinics in order to achieve optimal PGT results and can assist with troubleshooting. Problems are almost always solved after simple and easy to implement changes to procedure.
A detailed explanation of the PGT[M]Seq method and its requirements is given in the Juno Genetics User Manual. In brief, Juno can begin work on a new PGT[M]Seq test once it has received the following: 1) a genetic report describing the mutation(s) responsible for the disorder for which PGT[M]Seq is being requested; 2) blood samples from the couple requesting PGT[M]Seq (both the man and the woman, even if only one of them carries a mutation); 3) where possible a sample from a child or other close relative who has been tested for the mutation (blood, DNA or saliva); 4) a completed Requisition Form, confirming the request for PGT[M]Seq and providing relevant information concerning the patients. In rare cases, PGT[M]Seq is not technically possible. Additionally, PGT[M]Seq often requires several weeks in order to develop and validate a new test. For these reasons, patients should not begin a cycle until Juno has confirmed that a PGT[M]Seq test has been successfully developed.
Many of the advanced PGT methods used by Juno Genetics are available nowhere else in the world. Consequently, it is necessary to send samples to the Juno laboratory (in Oxford, UK) in order to access the tests. In most cases, the time required for the shipping of samples, and for the complex laboratory procedures, means that embryos must be cryopreserved (vitrified) while the test is carried out. Embryos can usually be thawed and transferred during the following cycle.
